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Image Search Results
Journal: Biomaterials advances
Article Title: In vitro development and optimization of cell-laden injectable bioprinted gelatin methacryloyl (GelMA) microgels mineralized on the nanoscale.
doi: 10.1016/j.bioadv.2024.213805
Figure Lengend Snippet: Fig. 5. Osteocyte morphology and functionality in mineralized and non-mineralized microgels. A-B and E-F represent the confocal images of non-mineralized (A-B) and mineralized (E-F) samples. Microgels stained with actin (green), DAPI (blue), and SOST (red) and SOST intensity (normalized fluorescence per cell) in mineralized microgels (A-H, K-L). Also, the dendrite length in μm (I) and the number of dendrites per cell (I-J) were not significantly different between mineralized and non-mineralized samples. Statistical differences are represented by **** p < 0.0001 after the One-way ANOVA test post-Turkey’s corrections.
Article Snippet: Cells were permeabilized with 0.1 % Triton X100, blocked with 1.5 % bovine serum albumin in PBS, and incubated at room temperature for 1 h. Samples were then washed in PBS and incubated with the primary antibody (rabbit M.G. da Costa Sousa et al. Biomaterials Advances 159 (2024)
Techniques: Staining, Fluorescence